Part:BBa_K3453142
Rosewood DmRbcL Trigger 1.2 transcription unit
This part is a transcription unit of the Rosewood DmRbcL Trigger 1.2 (BBa_K3453042) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).
Usage and Biology
A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA thereby exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).
Figure 1. Toehold switches principle.
This part is a transcription unit of BBa_K3453042 which is a trigger for the Rosewood DmRbcL Toehold Switch 1.2 (BBa_K3453032) and also contains the switch binding site for the Rosewood DmRbcL Toehold Switch 1.1 (BBa_K3453031).
This part proved to be functional: the transcribed rosewood RNA was able to bind to the Rosewood DmRbcL Toehold Switch 1.1 and a readable output was generated. However, the Rosewood DmRbcL Toehold Switch 1.2 turned out to be not functional and the interaction with this switch could not be assessed.
Full results are available on the BBa_K3453131 and BBa_K3453132 pages in the registry.
References
[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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